内皮素对血管平滑肌细胞中—氧化氮合成酶基因表达的影响

Effect of Endothelin on Expression of Nitric Oxide Synthetase of Vascular Smooth Muscle Cell Gene

摘要: 应用反转录－聚合酶链式反应（reversetranscription－polymerasechainreaction，RT-PCR）方法，测定出一氧化氮合成酶（nitricoxidesynthetase，NOS）mRNA不仅在心血管系统细胞中广泛存在，并且发现内皮素可以促进血管平滑肌细胞中NOSmRNA的表达（＋34．8％），增高血管平滑肌细胞中NOS酶活性（＋74％）。结果提示，由内皮素引起的血管平滑肌细胞中NOS基因表达和NOS活性的增加，可能是高血压中的一个重要的负反馈因素。

Abstract: With reverse transcripti0n-polymerase chain reaction （RT-PCR）,it has been found that NOS mRN;\ is widely distributed in cells of car＜liovascular system such as t’ardiomyocytes, endothelia1 cels, vascular smooth musclc cel1s （VSMC ）. When ET （1O-’ mol/L ） was added to cu1tured VSMC, it significant1y increased the NOS mRNA leve1 in VSMC （+ 34. 8% ）. By measuring the enzyme activity of NOS in cultured VSMC,it was f0und that when VSMC was treated with ET （2 X 10wv mol/L） l NOS activati0n was significantly increased （79% ） compared to the contr0l. These data show that the increased expression of NOS gene inVSMC induced by ET may be an important regulat0ry factor for hypertension.