Abstract: Aim\ In ordor to screen cardiovascular associated genes from a human fetal heart（HFH） cDNA library,the express sequence tags（ESTs） were detected with automatic DNA sequencer.\ Methods\ （1）To construct HFH cDNA library,the total RNA was extracted from fetal heart.（2）Picking up plaques to prepare sequencing templates,the cDNA inserts was determined by PCR in the presence of vector primers flanking the inserts（using T 3 as forward and T 7 as reverse primer）.（3）The PCR products were used directly for cycles sequencing using a fluorescence conjugated T 3B primer.（4）Following sequence generation using DNA sequencer.（5）The ESTs were compared with all sequences in Gene bank data bases.\ Results\ （1）The cDNA library titer was 1×10 9, the inserts were approximztely 12￣15 kb;the positive clones were more then 70%, the length of cDNA sequences were 210￣550 bp（average 300 bp） by electrophoresis of 6% ureapolyacrylamide gel in 5 hours;（2）The new sequences（or ESTs） were 474% and the known genes were 441% among 3132 ESTs.\ Conclusion\ This study shows that the method is useful to screen and identify cardiovascular associated genes by ESTs from HFH cDNA library, screening ESTs is ensured successfuly in construction of high quality cDNA library.