Abstract: Objective\ To explore the association of the Arg64 polymorphism β\-3\|adrenergic receptor（β 3\|AR） gene with polycystic ovary syndrome（PCOS）and its characterization with obesity,insulin resistance（IR）,blood pressure （BP）, blood lipid spectrum. Methods\ The polymorphism of the β\-3\|AR gene was determined in 163 patients with PCOS and 100 controls. Body mass index（BMI）, waist to hip circumference ratio（WHR）, BP were examined. Blood glucose,blood lipid spectrum,insulin were also measured. Insulin resistance index was calculated. Results\ The frequency of Arg64 allele in PCOS women was significantly higher than that of controls（0.33 vs 0.13 respectively, P ＜0.05）. Fasting insulin, insulin resistance index, WHR in Arg64 group were significantly higher than those of Trp64 group. The frequency of Arg64 allele was positively correlated with FINS, insulin resistance index, WHR. Conclusion\ The Arg64 mutation in the β\-3\|adrenergic receptor（β\-3\|AR） gene might contribute to development of PCOS and IR.