Abstract: Objective\ To study the proliferation and migration of vascular smooth muscle cell （VSMC） after transferred angiotensin Ⅱ （AngⅡ） type 2 receptor （AT 2R） gene. Methods\ The recombinant adenoviral vector, AdCMV\|AT\-2R, containing rat AT\-2 receptor gene was constructed by homologous recombination, and transfered to rat VSMC in vitro. The expression of AT\-2R mRNA was detected by RT\|PCR. The rate of expression and the change of cell cycle in VSMC were analysed by flow cytometry. These assays including cell devision index. Incorporation of bromodeoxyuridine（BrdU） and 3\|（4,5\|dimethyl\|thiazol\|2\|yl）2,5\|diphenyltetrazolium bromide（ MTT） were used to determine the proliferation of VSMC. The modified Boyden’s chamber method was used to test the migration of VSMC. The re\|organization of F\|actin in VSMC was analysed by confocal microscopy. Results\ RT\|PCR showed that the expression of AT\-2R mRNA increased substantially in transferred VSMC, and the peak value of expression rate is about 89.51% at 48 hours. When the expression of AT\-2R is at peak value, the ratio of S, G\-2 and M periods was reduced from 31.7% to 13.9%（\%P＜\%0 05）. The OD values of MTT and BrdU incorporation were reduced by 61.4% and 51.6% respectively(\%P\%＜0 0（1）. the number of VSMC migration was reduced by 62.2%（\%P\%＜0 05） and the expression of F\|actin was also decreased significantly. Conclusion\ AdCMV\|AT\-2R induced high level expression of AT\-2 receptor in cultured rat VSMC and its expression can significantly inhibit the proliferation and migration of rats VSMC in vitro .