心肌梗塞后大鼠心肌组织中神经肽Y Y1受体mRNA水平的改变

Changes in Neuropeptide Y Y1 Receptor mRNA Level in the Infarcted Myocardial Tissues of Rats

    摘要
  • 摘要: 目的　观察心肌梗塞后心肌组织中神经肽Y(NPY)Y1受体水平的改变。方法　实验在雄性Sprague Daw ley大鼠中进行 ,用结扎大鼠冠状动脉左前降支的方法 ,造成心肌梗塞动物模型 ,用RT PCR方法观察心肌组织中是否存在有NPYY1受体mRNA ,用定量PCR方法观察心肌梗塞后大鼠心肌组织中NPYY1受体mRNA水平的改变。结果　用RT PCR方法观察到 ,大鼠心肌组织中有NPYY1受体mRNA分布。在大鼠结扎冠状动脉一天及三天后 ,梗塞区及非梗塞区心肌组织中NPYY1受体mRNA水平较假手术组大鼠均有显著升高。此外 ,假手术组大鼠心肌组织中NPYY1受体mRNA水平较正常大鼠也有显著升高。结论　心肌梗塞可导致梗塞区及非梗塞区心肌组织中NPYY1受体水平升高 ,手术创伤应激也可影响心肌组织中NPYY1受体水平。

     

    Abstract: Aim\ To investigate the change in neuropeptide Y（NPY） Y\-1 receptor level in infarcted myocardium tissues of （MI） rats. Method\ MI was induced by ligating the left descending anterior coronary artery （LAD） in the heart of rats. The techniques of reverse transcription polymerase chain reaction （RT\|PCR） were used to identify the exist of NPY Y\-1 receptor mRNA in myocardial tissues. The technique of semi\|quantitative PCR was used to observe the change of NPY Y\-1 receptor mRNA level. Results\ NPY Y\-1 receptor mRNA distributed in the heart tissues of rat. Compared with sham operated rats ,the NPY Y\-1 receptor mRNA level both in infarction area and non\|infarction area was increased significantly after MI for 1d and 3d. The NPY Y\-1 receptor mRNA level in the heart tissues of sham\|operated rats was also significantly increased compared with that in control rats. Conclusion\ These results suggest that MI may result in increase NPY Y\-1 receptor level in the heart tissues of rats. Stress stimulation such as surgery may also increase the NPY Y\-1 receptor level in the heart.

     

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