寒冷应激和高盐饮食复合环境因素对大鼠血压的影响

Effect of Two Environmental Factors on Blood Pressure in Rats

  • 摘要: 目的 应用寒冷刺激及高盐饮食复制大鼠高血压模型 ,探讨环境因素在高血压发病中的作用。方法  10 0只3月龄的雄性大鼠 ,随机分成 4组 :1.寒冷组 (n =2 8) ,动物置于 4± 2℃的冷环境 ,每天 4小时 ;2 .高盐组 (n =2 8) ,每天给予 8%的盐饲料 ;3.复合组 (n =2 8) ,动物在暴露于冷环境的基础上每天还给予 8%的高盐饮食 ;4.正常对照组 (n=16 ) ,不给任何刺激因素。四组总的实验时间为 8周。每周测一次尾压、心率。结果 第一周末 ,寒冷、高盐、复合组的尾压、心率比对照组均明显升高 (P· <0 0 1) ,三周末血压达到最高 ,分别为 (12 7.0± 6 .0 ,12 5 .9± 5 .5 ,131± 6 .0 )mmHg。 结论 应用复合环境因素成功地复制了大鼠高血压模型 ;环境因素对血压有明显影响。

     

    Abstract: Objective\ 1.To explore the role of environmental factors in the development and maintenance of hypertension . 2. To establish a hypertensive model without surgical or pharmacologic intervention. Methods\ One hundreds male, 2\|3\|month\|old Wistar rats were randomly divided into 4 groups: 1. Cold\|treated group(C, \%n\%=28): animals were exposed to cold (4±2)℃ for 4 hours per day for 8 weeks; 2. High salt\|treated group (S, \%n\%=28): animals were given 8% NaCl diets for 8 weeks. 3. Cold combined high salt\|treated group (CS, \%n\%=28).\ Animals were given 8% NaCl diets for 8 weeks besides cold exposure. 4. Control group (N, \%n\%=16). Systolic blood pressure(SBP, Tail\|cuff technique) and heart rate were measured weekly in 4 groups throughout the experiment. The thoracic aortas were extracted for morphologic studies. Results\ On the end of the first week, the average SBP and heart rate of C, S, and CS group were sighificantly increased (\%P\%<0 01) than that of the N group. Blood pressure reached a maximal level by 3 weeks in C, S, and CS group (127.0±6.0, 125.9±5.5, 131±6.0)mmHg, respectively, SBP remained significantly elevated for the remaining of the experiment. Conclusion\ Cold circumstance and high\|salt intake play an important role in the development of hypertension. This model appears to be a successful model for the induction of hypertension in rats without surgical intervention or excessive doses of hormones.

     

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