Abstract: Objective To determine whether vasorelaxation induced by amlodipine depend on eNOS activation. Method A constant perfusion model of rabbit femoral artery was used to observe the changes of perfusion pressure before and after intra arterial infusing 10 -3 mol·L -1 amlodipine 0 5 ml and the effect of NOS inhibitor L NAME on this action. Result Relaxation of rabbit femoral artery bed induced by amlodipine was partialy inhibited by NOS inhibitor L NAME. Conclusion Vasorelaxation induced by amlodipine is mediated partialy through eNOS activation.