Abstract: Objective To study the relationship between gender,age and radial augmentation index（rAI）. Methods Two thousands four hundreds and fifty six healthy subjects were recruited 1192 males and 1264 females,mean age （43.1±13.4）years,radial arterial waveforms were obtained by radial tonometry Colin HEM-9000AI. Results The average value of rAI in Chinese healthy subjects was （74.2±15.0）%. Radial AI was significantly higher in women than that in men （79.5±13.1）% vs （68.6±14.9）%,P＜0.01. Radial AI was positively related to age in healthy men and women （men:r=0.526,women:r=0.545,P＜0.01,respectively）. Conclusion rAI seemed to be related with age and gender in healthy subjects,with higher value in women,emphasizing normal value of rAI should be determined based on age range.