Abstract: Objective To examine the detection efficiency of radioimmunoassay（RIA）and chemiluminescence immunoassay（CLIA）methods in measuring plasma renin and aldosterone for screening primary aldosteronism（PA）.Methods Plasma renin and aldosterone were measured in 96 patients with hypertension by RIA and CLIA methods,respectively. The correlation between the two methods was analyzed. The area under curve（AUC）,as well as the sensitivity and specificity of the two methods were computed by receiver operating characteristic curve（ROC）.Results Forty-one patients were diagnosed with PA. The correlation between the plasma renin and aldosterone concentration detected by two methods was positively correlated（r=0.905 and 0.967,respectively,both P＜0.01）.The AUC of ROC for the aldosterone to renin ratio（ARR）determined by RIA and CLIA methods screening PA were 0.94（95% confidence interval or CI 0.89-0.99）and 0.92（95% CI0.85-0.98）,respectively,with no significant differences（P＞0.05）. The sensitivity and specificity were 90.2% and 92.7%for RIA method at the cutoff point of 30（nmol/L）/ng/（L·h）for ARR,and 95.1%and 83.6%for CLIA method at the cut-off point of 25 for ARR. The sensitivity and specificity of the parallel test were 97.6% and 83.6%. Conclusion Both RIA and CLIA methods are closely correlated in measuring plasma renin and aldosterone.Compared with RIA method,CLIA method has a relatively higher sensitivity but lower specificity in screening PA.