Abstract: Objective To investigate the effect of acute exercise on the levels of circulating endothelial microparticles（EMPs）in patients with well-controlled essential hypertension. Methods Forteen male essential hypertensive patients with well-controlled blood pressure aged（56.4±5.0）yearsand 12 male normotensive volunteers aged（55.3±7.2）yearswere enrolled. Flow cytometry was used to detect the levels of circulating EMPs. Hypertensive patients received a single moderate intensity aerobic exercise referring to the modified Bruce’s method for 30 min.The levels of circulating EMPs were compared before and after exercise. Results The levels of blood pressure（BP）between hypertensive patients and normotensive volunteers were no statistically different. Compared with normotensive volunteers,the concentration of circulating EMPs was elevated in BP well-controlled hypertensive patients（1842.4±308.3）vs（922.5±136.4）counts/μL,P＜0.01. In those hypertensive patients,the level of circulating EMPs was attenuated after exercise compared with the resting state （1668.8±264.5）vs（1842.4±308.3）counts/μL,P＜0.05. Conclusion The level of circulating endothelial microparticles is significantly higher in well-controlled essential hypertensive patients than that in normotensive volunteers. And it is alleviated after exercise,which suggesting that exercise can further improve the impaired endothelial function in hypertensive patients whose boold pressure is well-controlled by antihypertensive drugs.