Abstract: Objective To investigate the interaction of angiotensinogen（AGT）gene 7loci on susceptibility to essential hypertension（EH）. Methods A total of 400 EH patients and 100 controls were recruited. The genotypes of AGT gene 7loci were determined by improved multiple ligase detection reaction（imLDR）. The interaction of 7loci for EH risk was analyzed by multifactor dimensionality reduction（MDR）method. Results There were significant differences in AGT gene G217A（CC:66.0%in EH patients vs 59.0%in controls）,A6C（TT:69.8% vs 57.0%）,C+31T（GG:68.0% vs 57.0%）,C4027T（GG:68.8% vs 58.0%）and C3889T（AA:0.3% vs 4.0%）polymorphisms（all P＜0.05）. The best diagnostic model was the interaction of AGT gene C3889 T,A6C,G217 Aand C+31Tpolymorphisms（odds ratio,95%confidence interval:1.86,1.19-2.92,P＜0.01）. Conclusion The interaction of multiple loci in AGT gene played a synergistic role in the development of EH.