闽东地区畲族成年人H型高血压与亚甲基四氢叶酸还原酶基因 C677T多态性的关联

Association between H-type hypertension and methylenetetrahydrofolate reductase gene C677T polymorphism in She adults in East Fujian

    摘要
  • 摘要: 目的 探讨闽东地区畲族成年人H型高血压与亚甲基四氢叶酸还原酶（MTHFR）基因C677T多态性的关联性。方法 选取福建省宁德市闽东医院2019年10月至2022年8月在高血压门诊就诊、住院和进行健康体检，且三代内未与其他民族通婚、居住在福安市境内的畲族成年人共309人为受试者。根据血压和同型半胱氨酸（Hcy）水平分为4组：Hcy正常非高血压组58例；Hcy升高非高血压组59例；Hcy正常高血压组77例；H型高血压组115例。Hcy≥10μmol/L定义为升高。采用Sanger测序法检测MTHFR基因C677T多态性，生化分析仪测定Hcy水平。比较4组患者MTHFR基因C677T多态性的差异，通过logistic回归分析MTHFR C677T基因多态性与H型高血压的关联。结果 Hcy正常非高血压组中MTHFR C677T基因CC、CT、TT基因型频率分别为70.7%、25.9%、3.4%;Hcy升高非高血压组中分别为54.2%、42.4%、3.4%;Hcy正常高血压组中分别为51.9%、41.6%、6.5%;H型高血压组中分别为36.5%、47.8%、15.7%。H型高血压组CT、TT基因型频率高于其他3组，而CC基因型频率低于其他3组（χ2=24.790,P<0.001）;其他3组基因型频率差异无统计学意义（χ2=6.004,P=0.199）。H型高血压组不同基因型患者Hcy差异有统计学意义TT基因型21.15（14.22～28.40）比CT基因型15.10（12.00～17.30）比CC基因型15.40（12.75～16.60）μmol/L,Z=9.866,P=0.007。多因素logistic回归分析显示，MTHFR基因C677T TT基因型发生H型高血压的风险增加OR（95%CI）=2.666（1.719～4.134）。结论 闽东地区畲族成年人H型高血压与MTHFR基因C677T多态性可能存在关联。

     

    Abstract: Objective To discuss the relationship between H-type hypertension and methylenetetrahydrofolate reductase（MTHFR） gene C677T polymorphism in She minority adults in East Fujian. Methods Between October 2019 and August 2022, a total of 309 She adults who had not married with other ethnic groups within three generations living in Fu’an, Ningde City were selected from hypertension outpatients, inpatients in MinDong Hospital. According to the blood pressure and the level of homocysteine（Hcy）, the subjects were divided into four groups: normal Hcy non-hypertensive group（n=58）, elevated Hcy non-hypertensive group（n=59）, normal Hcy hypertension group（n=77）, H-type hypertension group（n=115）. Hcy≥10 μmol/L was defined as elevated. The MTHFR C677T polymorphism was detected by Sanger sequencing. Hcy was determined by biochemical analyzer. The differences of MTHFR C677T gene among 4 groups were compared. Association between MTHFR C677T gene polymorphism and H-type hypertension was analyzed by logistic regression analysis. Results The MTHFR C677T gene CC, CT and TT genotypes frequencies were 70.7%, 25.9% and 3.4%, respectively in normal Hcy non-hypertension group; 54.2%, 42.4% and 3.4%, respectively in elevated Hcy non-hypertension group; 51.9%, 41.6% and 6.5%, respectively in normal Hcy hypertension group; 36.5%, 47.8% and 15.7%, respectively in H-type hypertension group. The frequencies of CT and TT genotypes were higher, while the frequencies of CC genotypes were lower in H-type hypertension group than those in the other three groups（χ~2=24.790, P＜0.001）, and there was no statistically significant difference among other 3 groups（χ~2=6.004, P=0.199）. There was significant difference for Hcy in H-type hypertension patients with different genotypes TT 21.15（14.22-28.40） vs CT 15.10（12.00-17.30） vs CC 15.40（12.75-16.60） μmol/L, Z=9.866, P=0.007. Multivariate logistic regression analysis showed that TT genotype was associated with increased risk of H-type hypertension OR（95%CI）=2.666（1.719-4.134）. Conclusion The C677T polymorphism of the MTHFR gene may be associated with the prevalence of H-type hypertension in She minority adults in the East Fujian area.

     

    • HTML全文
    • 参考文献(25)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回